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Protein-protein interactions (PPIs) play key roles in the cellular function and their dysregulation often leads to disease states. Here, we present a novel approach for the visualization of PPIs utilizing the click chemistry approach. The approach is based on the selective ligation of a small-molecule reporter to a specific protein domain. We demonstrate the selective labeling of the domain containing the ligand-binding site and the simultaneous labeling of associated interacting partners by enzyme-based ligation of a small molecule-derived reporter. The developed system allows for the visualization of multiple PPIs in living cells.The National Security Agency has ordered all its employees at every location to destroy what they currently have in their possession, a request that the agency plans to begin following within the next month.
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The document, a directive from the NSA's deputy director for operations, general counsel, and chief information officer, reveals a growing paranoia within the NSA that those possessing information about its illegal activities, whether whistleblowers, Congress or its own employees, might be compelled to give it up.
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